• Robust fluorescent probe qPCR & PCR performance
  • Applicable to wider range of probe designs and detection chemistries
  • Superior performance with pure template
  • Overcomes many known PCR inhibitors



Common PCR inhibitors and their source:

InhibitorsCommon Source
Polysaccharides Plants, seeds, stool
Heme, hemoglobin Blood
Humic acid Soil, plant material
Melanin Skin, hair

Popular ToughMix® Products

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  • UltraPlex 1-Step ToughMix An advanced 4X concentrated master mix that enables highly sensitive 1-step RNA detection (RT-qPCR) using multiplexed probe-based real-time PCR with crude extracts or inhibitory biological starting materials
  • AccuStart Genotyping ToughMix An advanced 2X concentrated ready-to-use reaction cocktail optimized with destabilizing buffer chemistry to enable accurate SNP genotyping with crude extracts, clinical specimens, environmental samples, plant tissues, animal tissues, or complex food matrices
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    An advanced 5X concentrated ToughMix reagent optimized to support highly-multiplexed real-time qPCR amplification across a range of inhibitory sample types and crude extraction methods